FAQ: Are libraries that have been dual indexed using the NEBNext® LV Unique Dual Index Primers compatible with single-end sequencing?

Yes, libraries generated with the NEBNext LV Unique Dual Index Primers are compatible with single-read sequencing. Please refer to Illumina’s Indexed Sequencing Overview Guide (current version) for details on dual-indexed sequencing workflows on a single-read flow cell or a paired-end flow cell.