HomeFAQsWhen using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110), upon ethanol addition to the flow-through, the sample looks cloudy. Is this a problem?
FAQ: When using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110), upon ethanol addition to the flow-through, the sample looks cloudy. Is this a problem?
It is normal to see some cloudiness when ethanol is added to the flow-through when processing certain sample types. This is a transient phenomenon that disappears after gentle but thorough pipette mixing and does not negatively impact the kit’s performance or the final result.
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