HomeFAQsCan the genomic DNA captured on the Monarch Spin Columns S2C (NEB #T3017) be eluted and used in downstream applications?
FAQ: Can the genomic DNA captured on the Monarch Spin Columns S2C (NEB #T3017) be eluted and used in downstream applications?
Yes. Although the kit was not developed for this specific application, the genomic DNA can be eluted from the Monarch Spin Columns S2C for use in other workflows. Please remember to wash the column once with Monarch Buffer BX, followed by twice with Monarch Buffer WZ , before elution with nuclease-free water or TE buffer. Note that NEB has not performed extensive characterization of the DNA recovered from the Column S2C. Customers are encouraged to evaluate the DNA in accordance with their intended downstream application.
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