The specific activity of SalI-HF in the recommended NEBuffer 4 is 2.3 x 106 Units/mg. Comparatively, the specific activity of SalI- HF™ in NEBuffer 4 + BSA is 4.6 x 106 Units/mg. The specific activity of SalI wild type in the recommended NEBuffer 3 + BSA is 4.5 x 106 Units/mg using lambda HindIII digest as the substrate.
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