FAQ: What is the activity of T7 Endonuclease I at different reaction temperatures?

0°C: some nicking
25°C: some nicking
37°C: 10,000 units/ml
45°C: 20-40,000 units/ml
65°C: 10-20,000 units/ml
80°C: 6,000 units/ml
90°C: 500 units/ml
The literature reports that the enzyme loses its ability to detect insertions or deletions at higher temperatures.