
RNA-seq
Choose Type:
- Batch Method Protocol (S1560) for Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
- Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
- NEBNext® Magnesium RNA Fragmentation Module Protocol
- Protocol for NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (E6111)
- Validate the Library - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (For use with NEB #E7300, #E7330, and #E7580)
- Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
- Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)
- NEBNext Multiplex Small RNA Sample Prep Set for Illumina - Library Preparation (E7330)
- Protocol for NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550)
- Protocol for Use with Previously Fragmented RNA - NEBNext mRNA First Strand Synthesis Module (E7525)
- Protocol for Use with Total RNA - NEBNext mRNA First Strand Synthesis Module (E7525)
- Library Preparation - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7580)
- QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7300)
- QC Check and Size Selection using 6% PolyAcrylamide Gel (E7330)
- QC Check and Size Selection using AMPure XP Beads - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7300)
- QC Check and Size Selection using AMPure XP Beads (E7330)
- QC Check and Size Selection Using Pippin Prep - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7300)
- Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using DNA Polymerase I, Large (Klenow) Fragment (M0210)
- Protocol for blunting ends by 3’ overhang removal and 3’ recessed (5’ overhang) end fill-in using T4 DNA Polymerase (M0203)
- Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
- Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB #E7490 and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530)
- Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420)
- Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420)
- Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat), NEB #E6310 and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530)
- Please see manual (NEB #E6100) for protocols
- Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.
- Please see manual (NEB #E7535) for protocols
- Protocol for use with Purified mRNA or Ribosome Depleted RNA - NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420)
- Protocol for use with Purified mRNA or Ribosome Depleted RNA - NEBNext Ultra RNA Library Prep Kit for Illumina (E7530)
- QC Check and Size Selection Using Pippin Prep (E7330)
- RNA Synthesis Brochure
- RNA Technical Guide
- Getting Started with RNA-Sequencing (RNA-Seq)
Brochures
Usage Guidelines
- Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S (2019) Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J Biol Chem; 294(48), 18220-18231.. PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.