Genome Editing Selection Chart
Programmable Nucleases Selection Chart
Cas9 Nuclease, S. pyogenes | EnGen® Lba Cas12a (Cpf1) | EnGen® Sau Cas9 | EnGen® Seq1 Cas9 | EnGen® Spy Cas9 HF1 | EnGen® SpRY Cas9 | EnGen® Spy Cas9 Nickase | EnGen® Spy Cas9 NLS | EnGen® Spy dCas9 (SNAP-tag®) | Authenticase® | T7 Endonuclease I | EnGen® Mutation Detection Kit | EnGen® sgRNA Synthesis Kit, S. pyogenes | |||
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GENOME EDITING APPLICATIONS | |||||||||||||||
In vitro digestion of DNA | *** | ** | ** | ** | ** | *** | **(x) | ** | |||||||
Transfection of RNP directly into cells | * | *** | *** | *** | *** | *** | *** | *** | |||||||
Disruption of gene of interest via NHEJ application | * | *** | *** | *** | *** | *** | |||||||||
High fidelity genome editing | *** | ||||||||||||||
Longer PAM for reduced off-target effects | *** | *** | ** | **(x) | |||||||||||
Programmable site-specific DNA nicking for genome editing and in vitro applications | *** | ||||||||||||||
Wide temparature range for enzyme activity | *** | *** | |||||||||||||
Visualization and target enrichment via SNAP-tag for attachment of fluorophores, biotin, and a number of other conjugates. | *** | ||||||||||||||
Target AT-rich regions | *** | ** | |||||||||||||
Collateral
Cleavage (trans cleavage)
activity. Target Detection |
*** | ||||||||||||||
Contains NLS (nuclear localization signal) | *** | *** | *** | *** | ** | *** | *** | *** | |||||||
GUIDE RNA SYNTHESIS APPLICATIONS | |||||||||||||||
Synthesize µg quantities of guide RNA for Spy Cas9 | *** | ||||||||||||||
MUTATION DETECTION APPLICATIONS | |||||||||||||||
Determination of the targeting efficiency of genome editing protocols (>2nt mismatch) | ** | ** | *** | ||||||||||||
Determination of the targeting efficiency of genome editing protocols (single base pair mismatches) | *** |
Key | |
*** | Optimal, recommended nuclease for this application |
** | Works well for selected application |
* | Will perform selected application, but is not recommended |
(x) | When using two RNA guides, one targetting each strand |