Genome Editing Selection Chart

Programmable Nucleases Selection Chart

  Cas9 Nuclease, S. pyogenes EnGen® Lba Cas12a (Cpf1) EnGen® Sau Cas9 EnGen® Seq1 Cas9 EnGen® Spy Cas9 HF1 EnGen® SpRY Cas9 EnGen® Spy Cas9 Nickase EnGen® Spy Cas9 NLS EnGen® Spy dCas9 (SNAP-tag®)   Authenticase® T7 Endonuclease I EnGen® Mutation Detection Kit   EnGen® sgRNA Synthesis Kit, S. pyogenes
GENOME EDITING APPLICATIONS
In vitro digestion of DNA *** ** ** ** ** *** **(x) **              
Transfection of RNP directly into cells * *** *** *** ***   *** *** ***            
Disruption of gene of interest via NHEJ application * *** *** *** ***     ***              
High fidelity genome editing         ***                    
Longer PAM for reduced off-target effects   *** *** **     **(x)                
Programmable site-specific DNA nicking for genome editing and in vitro applications             ***                
Wide temparature range for enzyme activity   ***   ***                      
Visualization and target enrichment via SNAP-tag for attachment of fluorophores, biotin, and a number of other conjugates.                 ***            
Target AT-rich regions   *** **                        
Collateral Cleavage (trans cleavage) activity.
Target Detection
  ***                          
Contains NLS (nuclear localization signal)   *** *** *** *** ** *** *** ***            
GUIDE RNA SYNTHESIS APPLICATIONS
Synthesize µg quantities of guide RNA for Spy Cas9                             ***
MUTATION DETECTION APPLICATIONS
Determination of the targeting efficiency of genome editing protocols (>2nt mismatch)                     ** ** ***    
Determination of the targeting efficiency of genome editing protocols (single base pair mismatches)                     ***        


Key  
   
*** Optimal, recommended nuclease for this application
** Works well for selected application
* Will perform selected application, but is not recommended
(x) When using two RNA guides, one targetting each strand