RNA Extraction & Purification
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FAQs for RNA Extraction & Purification
- Is the Monarch® Total RNA Miniprep Kit compatible with Luna® RT-qPCR Reagents?
- Is the Monarch® Total RNA Miniprep Kit compatible with NEBNext® reagents for RNA Library Prep?
- What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
- Can I use the Monarch® Total RNA Miniprep Kit for RNA cleanup after enzymatic manipulations?
- Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from bacteria?
- Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from plants?
- Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from yeast?
- How can I preserve my sample if I am not ready to extract RNA right after harvest?
- I am seeing genomic DNA in my preps when using the Monarch Total RNA Miniprep Kit. What could be the problem?
- Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
- Can the Monarch Total RNA Miniprep Kit be used to purify RNA from drosophila?
- Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?
- Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal (cheek) swabs?
- Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
- Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from plasma or serum?
- Can I lyse my adherent cells directly in their wells when using the Monarch Total RNA Miniprep Kit?
- Can I do an on-column DNase I treatment with the Monarch RNA Cleanup Columns?
Protocols for RNA Extraction & Purification
- Batch Method Protocol (S1560) for Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
- cDNA synthesis on oligo (dT)25 magnetic beads (S1419)
- Isolation of mRNA from Mammalian Cells Using the Magnetic mRNA Isolation Kit (NEB #S1550)
- Isolation of mRNA from Tissue Using the Magnetic mRNA Isolation Kit (NEB #S1550)
- Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
- Protocol for isolation of siRNA from p19 siRNA Binding Protein (M0310) using chitin magnetic beads
- mRNA Isolation using Streptavidin Magnetic Beads (NEB #S1420 and NEB #S1421)
- Quantitation of Isolated Poly(A) RNA from the Magnetic mRNA Isolation Kit (NEB #S1550)
- Ligation protocol using SplintR® Ligase (M0375)
- Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Fractionation of Small and Large RNA Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- RNA Reaction Cleanup using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Samples in RNAlater® using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from TRIzol®-extracted Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
- FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit NEB T2010
- Quick Protocol for Total RNA Extraction (NEB #T2010)
- Extraction of RNA from Agarose Gels using the Monarch® RNA Cleanup Kits
- Monarch® RNA Cleanup Kit Protocol
- Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits
- Separation of Large and Small RNA into Fractions using the Monarch® RNA Cleanup Kits
- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Avoiding Ribonuclease Contamination
- Choosing RNA Input Amounts for NEB T2010
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidelines for Purification of RNA from Cultured Mammalian Cells
- Guidelines for RNA Purification from Bacteria
- Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
- Guidelines for RNA Purification from Plants
- Guidelines for RNA Purification from Tissues
- Guidelines for RNA Purification from Whole Blood
- Guidelines for RNA Purification from Yeast
- Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent
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Brochures
Troubleshooting Guides
Usage Guidelines
Manuals
Legal Information
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
