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PCR & DNA Cleanup

Monarch Spin PCR & DNA Cleanup solutions reliably purify highly concentrated, high-quality double-stranded and single-stranded DNA from PCR, restriction digestions and reverse transcription. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit ensure zero buffer retention and no carryover of contaminants, enabling elution in volumes as low as 5 µl. Monarch Buffers have been optimized, and do not require pH monitoring. Eluted DNA is ready for use in restriction digests, DNA sequencing, PCR, ligation, and other enzymatic manipulations. Purify small DNA fragments, including oligonucleotides, with this single kit, elute in as little as 5 µl, and complete the entire workflow in only 5 minutes. The kit produces more concentrated DNA with better DNA recovery and purity compared to the leading supplier!

 

Workflow pf DNA & PCR cleanup nucleic acid purification

 

 

Purify oligonucleotide DNA with a dedicated oligo cleanup protocol with Monarch Spin PCR & DNA Cleanup

 

Monarch Spin PCR & DNA Cleanup Kit (5 μg) effectively cleans up oligonucleotide DNA using the oligonucleotide cleanup protocol.

Graph showing Oligonucleotide Cleanup

Synthesized ssDNA (≥ 16 nt) and dsDNA (≥12 bp) oligonucleotides can be effectively purified and recovered using Monarch Spin PCR & DNA Cleanup Kit (5 μg). The provided oligonucleotide cleanup protocol was followed using 1 μg of oligonucleotides of varying lengths (8–25 nt/bp) as an input. DNA was eluted in 20 μl of Monarch Buffer EY. Concentrations of DNA were measured using a Trinean DropSense 16 and percent recovery calculations are based on the eluted DNA concentration and elution volume used. The minimum sized ss- and ds- oligonucleotides that can be used are marked with a star (*).



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FAQs for PCR & DNA Cleanup
Protocols for PCR & DNA Cleanup
Application Notes for PCR & DNA Cleanup
Product Manual
Advantages of the Monarch Spin PCR & DNA Cleanup Kit
  • Elute in as low as 5 µl for highly concentrated DNA
  • Prevent buffer retention and salt carry over with unique, optimized column design
  • Reduce hands-on time with faster protocols and less spin timeNo need to monitor pH
  • Protocol modification for oligonucleotide cleanup is provided, allowing purification of ssDNA, oligonucleotide and other small DNA fragments
  • Significantly less plastic by design in columns and kit compared to leading suppliers

Specification of the Monarch Spin PCR & DNA Cleanup Kit
Properties
Purification format Spin-column
DNA sample
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations)
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol
Typical recovery
70-90%
DNA Purity A260/280 > 1.8 and A260/230 > 1.8
Binding Capacity Up to 5 μg
Elution Volume 5-20 μl
DNA Size Range
Standard protocol: 50 bp – 25 kb
Protocol Time 5 minutes of spin and incubation time
Compatible Downstream Applications Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.