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Enrichment for Methylated DNA (5mC) and RNA (m6A)

Enrichment methods add efficiency to genomic and epigenomic studies. NEB offers kits and reagents that enable efficient downstream analysis of 5-Methylcytosine (5mC) modified DNA and N6-Methyladenosine (m6A) modified RNA species. Independently applicable, individual reagents complement the EpiMark® kits.

5mC DNA and m6A RNA species

Methylated DNA or RNA can be enriched for sequencing

  • EpiMark Methylated DNA Enrichment Kit (NEB #E2600) enriches and purifies double-stranded methyl-CpG from fragmented genomic DNA based on the selective and high affinity of the MBD2-Fc fusion protein and magnetic bead capture. Methylated and unmethylated DNA controls are included for qPCR.

  • EpiMark N6-Methyladenosine Enrichment Kit (NEB #E1610). N6-Methyladenosine antibody enables immunoprecipitation of m6A modified RNA using magnetic bead capture. Controls for N6-Methyladenosine modified and unmodified RNA are included for monitoring of enrichment and depletion with RT-qPCR.

  • Protein A Magnetic Beads (NEB #S1425) are compatible with methylated DNA immunoprecipitation.

  • Protein G Magnetic Beads (NEB #S1430) can be used in conjunction with the EpiMark® N6-Methyladenosine Enrichment Kit for isolation of enriched m6A RNA for downstream analysis by next-generation RNA sequencing or RT-qPCR.


 

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FAQs for Enrichment for Methylated DNA (5mC) and RNA (m6A)
Protocols for Enrichment for Methylated DNA (5mC) and RNA (m6A)
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

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    Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change.

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    In this episode we are talking about the importance of RNA modifications. Hear about protocols for prokaryotic transcription start site (TSS) determination, as well as detection and quantitation of RNA modifications, and learn how the RNA Modification Database(http://mods.rna.albany.edu) can help your RNA research.

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