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Epigenetic Analysis

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The EpiMark® line of products from New England Biolabs is designed to simplify DNA methylation and hydroxymethylation detection and analysis. This growing line of reagents and kits includes:

  • NEBNext E5hmC-seq Conversion Module (NEB #E3365) provides a high-performance enzyme-based method for detection of 5hmC at the single base level.
  • NEBNext® Enzymatic 5hmC-seq (E5hmC-seq™) (NEB #E3350) detects 5hmC using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC, after Illumina® sequencing.
  • EpiMark® Methylated DNA Enrichment Kit (NEB #E2600) to enrich double-stranded methyl-CpG from fragmented genomic DNA with as little as 5 ng of input DNA
  • EpiMark® Hot Start Taq DNA Polymerase (NEB #M0490) to amplifiy bisulfite-treated samples
  • EpiMark® 5-mC and 5-hmC Analysis Kit (NEB #E3317) to identify and quantify 5-hydroxymethylcytosine at specific loci
  • NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB #E7645) to prepare Chromatin immunoprecipitated DNA samples for next-generation sequencing
  • NEBNext® Enzymatic Methyl-seq (EM-seq™) (NEB #E7120), an enzyme-based alternative to bisulfite sequencing for sensitive 5mC and 5hmC detection using next generation sequencing

EpiMark® and NEBNext® are registered trademarks of New England Biolabs, Inc.
EM-seq™ is a trademark of New England Biolabs, Inc.
Illumina® is a registered trademark of Illumina, Inc.
SOLiD™ is a trademark of Life Technologies, Inc.

Choose Type:

Protocols for Epigenetic Analysis
Application Notes for Epigenetic Analysis
Types of Histone Modifications
Amino Acid Modification
Lysine Methylation, Acetylation,
Ubiquitination, Sumoylation,
ADP-Ribosylation
Arginine Methylation
Serine Phosphorylation
Threonine Phosphorylation
Types of DNA Modifications

References

  1. Kim, J.K., Samaranayake, M. and Pradhan S. (2009) Cell. Mol. Life Sci. 66, 596-612. PMID: 18985277
  2. Vanyushin, B.F. (2006) Curr. Top. Microbiol. Immunol. 301, 67-122. PMID: 16570846
  3. Mosher, R.A., Melnyk, C.W. (2010) Trends Plant Sci. 15, 204-210. PMID: 20129810
  4. Lyko, F., Beisel, C., Marhold, J., Paro, R. (2006) Curr. Top. Microbiol. Immunol. 310, 23-44. PMID: 16909905
  5. Selker, E.U., Freitag, M., Kothe, G.O., et al. (2002) Proc. Natl. Acad. Sci. U S A. 99, Suppl 4, 16485-16490. PMID: 12189210
  6. Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-930. PMID: 19372393
  7. Tahiliani, M., Koh, K. P., Shen, Y., et al. (2009) Science 324, 930-935. PMID: 19372391
  8. Ehrlich, M., Wilson, G.G., Kuo, K.C., And Gehrke, C.W. (1987) J. Bacteriol. 169, 939-943. PMID: 3029036
  9. Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A. and Matvienko, N.I. (2004) Biochem. (Moscow) 69, 299-305. PMID: 15061697
  10. Ratel, D., Ravanat, J-L., Berger, F. and Wion D. (2006) Bioessays 28, 309-315. PMID: 16479578
  11. Marinus, M.G. and Casadesus, J. (2009) FEMS Microbiol. Rev. 33, 488-503. PMID: 19175412
ChIP-Seq Library Construction Workflow
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • What is Epigenetics?

    What Is Epigenetics?

    If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

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