HomeFAQsHow many libraries can I quantitate at a time when using the NEBNext® Library Quant Kit for Ultima Genomics® (NEB #E3410)?
FAQ: How many libraries can I quantitate at a time when using the NEBNext® Library Quant Kit for Ultima Genomics® (NEB #E3410)?
We recommend running 2 dilutions of each library and running each dilution in triplicate during the development/optimization stage. Therefore, 12 libraries can be quantitate in a 96-well plate using 5 standards and 1 no-template control (NTC). Once a workflow is well established, a single dilution could be run, allowing quantitation of 24 libraries per 96-well plate using 5 standards and 1 NTC.
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