HomeFAQsDoes the library quant require a size correction when using the NEBNext® Library Quant Kit for Ultima Genomics® (NEB #E3410)?
FAQ: Does the library quant require a size correction when using the NEBNext® Library Quant Kit for Ultima Genomics® (NEB #E3410)?
Yes. The intercalating dye used in the NEBNext Library Quant Kit for Ultima Genomics binds DNA in proportion to the number of base pairs per double-stranded DNA product, so it is necessary to correct for product length by normalizing to the size of the DNA standard, 200 bp. All quants are simply multiplied by a correction factor, 200/library size. This is done automatically using our online tool for NGS Library Quant.
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