Endo-β-Galactosidase activity can be inhibited by ionic detergents, such as SDS, at moderate concentrations (0.5–1%). To counteract this inhibition, it is essential to include NP-40 at a final concentration of 1% in your reaction mixture.
In contrast, non-ionic detergents such as Tween 20, Triton X-100, and NP-40 can stabilize Endo-β-Galactosidase and enhance its specific activity.
