Yes, you can use a different buffer, as our Endo-β-Galactosidase (EBG) is active across a broad pH range (pH 3.5–9). However, optimal cleavage occurs near pH 6.0. At pH 8.0, activity decreases to less than 50%, and is even further reduced at pH 8.5 after 1 hour at 37°C in our standard assay.
To improve cleavage under non-optimal pH conditions, extend the incubation time, increase the enzyme amount, or both.
Additionally, buffer composition and salt concentration can affect efficiency. For instance, in our experiments, a 50 mM sodium phosphate buffer (pH 7.4) performed better than 1x PBS at the same pH.
