HomeFAQsWhat is the recommended concentration of Mg+2 to use with Bst-XT in LAMP reactions?
FAQ: What is the recommended concentration of Mg+2 to use with Bst-XT in LAMP reactions?
We recommend using a total of 6 mM MgSO4 in LAMP and RT-LAMP reactions with Bst-XT. The Bst-XT isothermal Amplification Buffer contains 2 mM MgSO4 at 1X, and therefore supplementing with additional 4 mM MgSO4 using the provided 100 mM MgSO4 (NEB #B1003) is recommended. If further optimization is necessary, the final MgSO4 can be increased up to 8 mM total.
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