FAQ: Can Bst-XT DNA Polymerase be used at temperatures other than 65°C for LAMP reactions?

Yes, Bst-XT can be used to perform LAMP reactions across the broad temperature range (50°C - 70°C). Optimal performance can vary between amplicons/primer sets, so screening temperatures may be beneficial. Use of Bst-XT is not recommended at temperatures greater than 72°C.