HomeFAQsCan Bst-XT DNA Polymerase be used in end point colorimetric detection?
FAQ: Can Bst-XT DNA Polymerase be used in end point colorimetric detection?
Yes, Bst-XT can be used in end point colorimetric detection using non pH-based metal indicator dyes including hydroxynaphthol blue (HNB). We recommend adding 120 µM HNB in 25uL LAMP/RT-LAMP reaction. When using HNB, the final MgSO4 concentration may be increased up to 8mM to enhance the color contrast between the positive and negative samples.
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