FAQ: Is it necessary to clean up RNA samples before RNase R (NEB #M0100) digestion?

Yes, it is necessary to clean up RNA samples before RNase R digestion, especially if the sample contains a high concentration of EDTA. RNase R is a magnesium-dependent exoribonuclease. We recommend that RNA samples contain less than 0.1 mM EDTA before RNase R treatment.