HomeFAQsHow long does the 3´ single-stranded RNA overhang need to be for RNase R (NEB #M0100) to bind?
FAQ: How long does the 3´ single-stranded RNA overhang need to be for RNase R (NEB #M0100) to bind?
RNase R prefers 3´ single-stranded RNA overhangs at least 10 nt in length. Longer overhangs, such as poly(A) tails, tend to result in higher RNase R activity. RNase R may not bind to overhangs that are shorter than 10 nt.
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