Home FAQs What conversion levels are typical with the control DNAs supplied in the EM-seq™ v2 kits?

FAQ: What conversion levels are typical with the control DNAs supplied in the EM-seq™ v2 kits?

Two DNA controls are provided in the EM-seq v2 kit: Unmethylated Lambda DNA and CpG-methylated pUC19 DNA. For 200 ng inputs, we observe ≤ 0.5% methylation, and for 0.1 ng inputs, we observe ≤ 1% methylation for unmethylated lambda spike in, indicating a conversion efficiency of  99.5% and  99%, respectively. For CpG-methylated pUC19, 96-98% CpG methylation is detected across the input range of 0.1-200 ng. As the pUC19 DNA is enzymatically methylated, it is likely that 100% methylation may not be achieved and that not all CpGs are methylated.