Home FAQs Are the NEBNext® LV Unique Dual Index Primers the same as NEBNext Multiplex Oligos for Illumina®?

FAQ: Are the NEBNext® LV Unique Dual Index Primers the same as NEBNext Multiplex Oligos for Illumina®?

No. The volume of the NEBNext LV Unique Dual Index Primers has been optimized for use with use with newer library prep kits, including the NEBNext Enzymatic 5hmC-seq Kit (NEB #E3350) and NEBNext Enzymatic Methyl-seq Kit V2 (NEB #E8015). NEBNext LV Unique Dual Index Primers products do not include an adaptor, and the required adaptor is supplied in compatible library prep kits.

The i5 and i7 unique dual indices associated with individual wells for the NEBNext LV Unique Dual Index Primers Set 3 (NEB #E3404S) are the same as those within the 96-well plate format of NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3) (NEB #E6444). The indices in NEBNext LV Unique Dual Index Primers Set 2B (NEB #E3392S) are a subset of NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2) (NEB #E6442).