HomeFAQsDoes RNase 4 Digestion and 3′ End Repair Mix (NEB #M1288) require magnesium?
FAQ: Does RNase 4 Digestion and 3′ End Repair Mix (NEB #M1288) require magnesium?
We recommend use of included NEBuffer r1.1 for RNA digestions with RNase 4 Digestion and 3′ End Repair Mix following our recommended digestion protocol. The 3′ end repair activity of T4 polynucleotide kinase requires magnesium for efficient phosphate removal. However, it is noteworthy that like RNase A, RNase 4 does not require magnesium for endoribonuclease activity.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
