HomeFAQsCan I use RNase 4 Digestion and 3′ End Repair Mix (NEB #M1288) for DNA-probe directed 5′ cap mRNA analysis?
FAQ: Can I use RNase 4 Digestion and 3′ End Repair Mix (NEB #M1288) for DNA-probe directed 5′ cap mRNA analysis?
We recommend dilution of RNase 4 (NEB #M1284) in our standard protocol for DNA-probe directed 5′ cap mRNA analysis. Implementation of this protocol with RNase 4 Digestion and 3′ End Repair Mix (NEB #M1288) will result in concurrent dilution of T4 PNK thereby impairing the efficiency of 3’-end repair. Therefore, we currently recommend using standalone RNase 4 (NEB #M1284) for DNA-probe directed 5’ cap mRNA analysis.
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