FAQ: Is there a recommended Ct value-range for RSV inputs?

NEB recommends inputs of at least 1,000 genome copies. As Ct values vary by assay type and the genome target of the assay, we do not have a specific Ct value range. However, as shown in the figure below sequencing of clinical samples with BioFire® assay Ct values of < 30 show high genome coverage. Refer to Figure 8 on the product page for data generated from clinical samples.