FAQ: Can I minimize non-specific barcode ligation amongst pooled samples, after using the NEBNext RSV Primer Module (NEB #E9642)?

Yes, add 2 ul of Thermolabile Proteinase K (NEB #P8111S) to each sample following the ONT barcode ligation reaction, then incubate at 37°C for 15 minutes. Following Thermolabile Proteinase K treatment, add 2 ul of EDTA provided in Oxford Nanopore Technologies Native Barcoding V14 Kit(s) to each barcoded sample and proceed with pooling and barcoded sample pool cleanup.