HomeFAQsCan I minimize non-specific barcode ligation amongst pooled samples, after using the NEBNext RSV Primer Module (NEB #E9642)?
FAQ: Can I minimize non-specific barcode ligation amongst pooled samples, after using the NEBNext RSV Primer Module (NEB #E9642)?
Yes, add 2 ul of Thermolabile Proteinase K (NEB #P8111S) to each sample following the ONT barcode ligation reaction, then incubate at 37°C for 15 minutes. Following Thermolabile Proteinase K treatment, add 2 ul of EDTA provided in Oxford Nanopore Technologies Native Barcoding V14 Kit(s) to each barcoded sample and proceed with pooling and barcoded sample pool cleanup.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
