Home FAQs Are there recommended RSV cDNA amplicon input (after using the NEBNext RSV Primer Module (NEB #E9642)) for Oxford Nanopore Technologies End Prep reactions for low-plexity or high-plexity library prep?

FAQ: Are there recommended RSV cDNA amplicon input (after using the NEBNext RSV Primer Module (NEB #E9642)) for Oxford Nanopore Technologies End Prep reactions for low-plexity or high-plexity library prep?

NEB recommends utilizing a minimum of 6 ONT Native Barcodes per sequencing run.  For runs utilizing 6 to 12 barcodes, we recommend inputs of 350 ng of amplicons within the 400-1,400 bp size range per sample going into the end prep reaction for ONT library prep.  

If utilizing more than 48 barcodes, 20 ng – 40 ng of amplicons within the 400-1,400 bp size range per sample can be used as input for the end prep reaction for ONT library prep.