Thermostable Endonuclease Q has activity in the following buffers (relative to activity in NEBuffer r2.1):
| NEBuffer r1.1 | 50% |
| NEBuffer r2.1 | 100% |
| NEBuffer r3.1 | 100% |
| rCutSmart Buffer | 75% |
| ThermoPol® Reaction Buffer | 100% |
| ThermoPol II (Mg-free) Reaction Buffer | 25% |
| LongAmp® Taq Reaction Buffer Pack | 75% |
| Isothermal Amplification Buffer | 50% |
| OneTaq Standard Reaction Buffer | 75% |
| Standard Taq Reaction Buffer | 50% |
| Q5® Reaction Buffer | 50% |
| Q5U® Reaction Buffer | 75% |
