HomeFAQsWhat is the difference between the suite of USER® enzymes (#M5505, #M5508, #M5509) and Thermostable Endonuclease Q (#M0701)?
FAQ: What is the difference between the suite of USER® enzymes (#M5505, #M5508, #M5509) and Thermostable Endonuclease Q (#M0701)?
The USER products (USER Enzyme (#M5505), Thermolabile USER II Enzyme (#M5508) and Thermostable USER III Enzyme (#M5509) are a mixture of two enzymes: UDG and an endonuclease. In contrast, Thermostable Endonuclease Q (#M0701) is a single enzyme. Although the USER enzymes and Thermostable Endonuclease Q both recognize and cleave at deoxyuracil in DNA substrates, the resulting products are different. The end products of the USER enzymes are one-nucleotide gaps with varying functional ends based on the type of USER implemented; the deoxyuracil is removed. The products of Thermostable Endonuclease Q are nicks with a 3′-OH and a 5′-phosphate; the deoxyuracil is still present on the 3′ segment of DNA.
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