FAQ: How does the unit definition for NEBExpress® Salt Active Nuclease (NEB #M0764) compare to other salt-active nucleases?

Although the unit definition assay conditions for NEBExpress Salt Active Nuclease differ (temperature, substrate, and buffer) from other similar nucleases on the market, we estimate that NEBExpress Salt Active Nuclease is up to 2 times more active compared to other salt nucleases. To switch from another salt nuclease to NEBExpress Salt Active Nuclease, we recommend testing a 1:1 substitution based on units.