Home FAQs When cloning a PCR fragment into the pMiniT 2.0 vector provided with the NEB PCR cloning kit, where in the multiple cloning site (MCS) of the vector is the insert ligated?

FAQ: When cloning a PCR fragment into the pMiniT 2.0 vector provided with the NEB PCR cloning kit, where in the multiple cloning site (MCS) of the vector is the insert ligated?

The insert will be ligated between nucleotides 545 and 546 of the pMiniT 2.0 vector. This insertion point is located between the recognition sites for EcoRi and PacI in the MCS region of the vector.