FAQ: Can this DNA polymerase be used in other NEBuffers for filling in or blunting overhangs?

FOR FILLING IN 5' OVERHANGS

DNA Polymerase I (E. coli), Large Klenow Fragment, Klenow Fragment (3'-5' exo-), and T4 DNA polymerase are all active in all NEBuffers, but NEBuffer 2/2.1/r2.1 provides optimal activity. Optimal activity of T7 DNA Polymerase is seen in its own buffer, and it has 50% reduced activity in the other NEBuffers. 
 
For comparisons of NEBuffer compositions, click here. To see the activity of more DNA modifying enzymes in rCutSmart Buffer, click here.

FOR BLUNTING OVERHANGS

NEBuffers DNA Polymerase I (E. coli) DNA Polymerase I, Large (Klenow) Fragment T4 DNA Polymerase T7 DNA Polymerase
1 Y N Y 50%
1.1 Y N Y 50%
 r1.1 Y N Y  50%
2 Optimal Optimal Y  50%
2.1 Y Y Y  50%
r2.1 Y Y Optimal   50%
3 Y Y N  50%
3.1 Y Y N  50%
r3.1 Y Y N  50%
4 Y Y Y  50%
CutSmart  Y Y Y  50%
rCutSmart  Y Y Y  50%
T4 DNA Ligase Buffer    Y Y  
T7 DNA Polymerse Reaction Buffer        Optimal 

"Y" means yes, this polymerase can be used in the specified buffer. "N" means no, this polymerase cannot be used in the specified buffer. Optimal buffers are indicated. Klenow (3'-5' exo-) cannot be used to remove overhangs.