HomeFAQsFor the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110), which culture media do you recommended for growing E.coli?
FAQ: For the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110), which culture media do you recommended for growing E.coli?
We suggest using LB (Luria-Bertani) media to grow E. coli. Rich media such as 2X YT or Terrific Broth can also be used, but typically result in significantly higher cell densities at saturation. Thus, it is necessary to scale back the amount of culture processed using rich media to avoid insufficient alkaline lysis and column overloading due to higher biomass.
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