FAQ: What kind of samples can be used with the NEBNext Immune Sequencing Kit (Mouse) (NEB #E6330)?

RNA from a variety of sources can be used with the NEBNext Immune Sequencing Kit (Mouse), including:

1. High quality RNA from B or T cells. The RNA should have a RIN score ≥7 to provide the highest percentage of full-length transcripts for library construction. When RNA is used with RIN scores <4, the complexity of the library is greatly reduced.
2. RNA from any mouse sample that contains either B or T cells, such as peripheral blood PBMC or tissue.
3. RNA extracted from frozen cell pellets, cultured cells, or cells sorted for specific B- or T-cell types.