FAQ: Why does the Luna Cell Ready Probe One-Step RT-qPCR kit contain more RT-qPCR reactions than lysis reactions?

The Luna Cell Ready Probe One-Step RT-qPCR kit pairs a lysis module containing 100 lysis reactions with a Probe One-Step RT-qPCR module of 500 reactions. This pairing is based on a typical experimental setup where a user may run multiple replicates of a transcript of interest (plus a reference) with appropriate no-RT controls. Conservatively, a 50 µl cell lysis reaction is sufficient at least for 40 RT-qPCR reactions assuming 1 µl lysate per reaction. Scale up lysis reactions accordingly if more RT-qPCR reactions are planned. Both the Luna Cell Ready Lysis Module (NEB #E3032) and the Probe One-Step RT-qPCR Kit (NEB #E3006) can be ordered separately if necessary.