All four polymerases are moderately thermostable DNA polymerases with strand displacement activity, enabling them to perform isothermal amplification reactions such as LAMP.
Bst 2.0 DNA Polymerase is an in silico designed homolog of Bst DNA Polymerase, Large Fragment. It is engineered for improved properties in LAMP reactions, including salt tolerance, thermostability, and dUTP incorporation. Bst 2.0 is notable for its high specificity.
Bst 3.0 offers a few improvements to Bst 2.0, most notably faster amplification speed. Bst 3.0 also has improved performance in higher temperature LAMP reactions (up to 72°C), yet for some targets Bst 3.0 does not retain the high specificity of Bst 2.0.
Bst-XT combines the most desirable properties of Bst 2.0 and Bst 3.0. It offers the high specificity of Bst 2.0 and the fast amplification speed of Bst 3.0. Bst-XT is also active across a broader temperature range, enabling LAMP reactions between 50-70°C.
| Bst-XT WarmStart NEB #M9204 |
Bst 2.0 WarmStart NEB #M0538 |
Bst 3.0 NEB #M0374 |
|
|---|---|---|---|
 Amplification Speed |
★★★★★ | ★★★ | ★★★★★ |
 Specificity |
★★★★★ | ★★★★★ | ★★ |
 Room temp. set-up? |
Enabled | Enabled | Not recommended |
| Optimal LAMP temp |
50-70°C | 60-70°C | 55-72°C |
 Available glycerol-free |
Yes NEB #M9205 |
Yes NEB #M0402 |
Yes NEB #M0443 |
