FAQ: The product sequence doesn't completely match the expected sequence. How can this result be improved?

Taq DNA Polymerase does not posess a proofreading function. To reduce the error rate use a polymerase that does, such as Q5® High-Fidelity DNA Polymerase (NEB# M0491) or Phusion® High-Fidelity DNA Polymerase (NEB# M0530). Q5 High-Fidelity DNA Polymerase (NEB #M0491) offers the highest fidelity available of any commercial thermostable polymerase.