NEBNext^® Quick Ligation Module

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E6056S     -20       NEBNext Quick Ligation Reaction Buffer E6058AVIAL -20 1 x 0.2 ml 5 X   Quick T4 DNA Ligase E6057AVIAL -20 1 x 0.1 ml Not Applicable
E6056L     -20       NEBNext Quick Ligation Reaction Buffer E6058AAVIAL -20 1 x 1 ml 5 X   Quick T4 DNA Ligase E6057AAVIAL -20 1 x 0.5 ml Not Applicable

1. NEBNext Quick Ligation Module Protocol (E6056)

• Tips for Maximizing Ligation Efficiencies

• DNA Ligase Selection Chart

1. What type of sample prep/library construction can I use the Quick Ligation Module reagents for?
2. How much DNA can I blunt in one NEBNext Quick Ligation Reaction?

• Troubleshooting Tips for Ligation Reactions

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E6056S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E6056L_v1_10011360
• E6056S_v1_10011244
• E6056L_v1_10015322
• E6056L_v1_10017869
• E6056S_v1_10016787
• E6056S_v1_10018358
• E6056L_v1_10022644
• E6056S_v1_10015754
• E6056L_v1_10015757
• E6056S_v1_10027721
• E6056L_v1_10029374
• E6056S_v1_10032127
• E6056L_v1_10025627
• E6056S_v1_10025624
• E6056S_v1_10036730
• E6056L_v1_10036733
• E6056S_v1_10044323
• E6056L_v1_10044928
• E6056L_v1_10047575
• E6056S_v1_10047669
• E6056L_v1_10041443
• E6056S_v1_10041440
• E6056L_v1_10058533
• E6056S_v1_10058334
• E6056L_v1_10061940
• E6056L_v1_10064505
• E6056S_v1_10058531
• E6056L_v1_10054714
• E6056S_v1_10065474
• E6056L_v1_10072789
• E6056L_v1_10072999
• E6056L_v1_10070786
• E6056L_v1_10075497
• E6056S_v1_10074718
• E6056L_v1_10071158
• E6056S_v1_10054711
• E6056S_v1_10082177
• E6056L_v1_10086966
• E6056S_v1_10071155
• E6056L_v1_10083004
• E6056S_v1_10097651
• E6056L_v1_10100455
• E6056S_v1_10101872
• E6056L_v1_10105406
• E6056S_v1_10094154
• E6056L_v1_10114114
• E6056S_v1_10112189
• E6056L_v1_10116314
• E6056S_v1_10116800
• E6056S_v1_10126615
• E6056L_v1_10107573
• E6056S_v1_10107570
• E6056L_v1_10135471
• E6056S_v1_10140201
• E6056L_v1_10141545
• E6056S_v1_10146803
• E6056S_v1_10154034
• E6056L_v1_10156119
• E6056S_v1_10162911
• E6056L_v1_10122405
• E6056S_v1_10122408
• E6056L_v1_10168098
• E6056L_v1_10171981
• E6056S_v1_10171482
• E6056S_v1_10175914
• E6056L_v1_10158780
• E6056S_v1_10180876
• E6056L_v1_10187339
• E6056S_v1_10158783
• E6056S_v1_10189570
• E6056L_v1_10198068
• E6056S_v1_10180435
• E6056S_v1_10209992
• E6056L_v1_10180432
• E6056S_v1_10219986
• E6056L_v1_10200560
• E6056S_v1_10200563
• E6056S_v1_10233256
• E6056L_v1_10226601
• E6056S_v1_10237990
• E6056S_v1_10226604
• E6056L_v1_10256094
• E6056S_v1_10258464
• E6056L_v1_10268594
• E6056S_v1_10266294

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext Quick Ligation Reaction Buffer
• Quick T4 DNA Ligase

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.