Gibson Assembly®
Reference:
1. Gibson, D.G., et al. (2009) Nat. Methods 6, 343-345.2. Gibson, D.G., et al. (2010) Science 329, 52-56.
Choose Type:
- PCR Protocol Phusion® DNA Polymerase
- Gibson Assembly® Master Mix – Assembly (E2611)
- Gibson Assembly® Protocol (E5510)
- Gibson Assembly® Chemical Transformation Protocol (E5510)
- Gibson Assembly® Chemical Transformation Protocol (E2611)
- Gibson Assembly® Electrocompetent Transformation Protocol (E2611)
- Gibson Assembly® Electrocompetent Transformation Protocol (E5510)
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
- Protocol for T5 Exonuclease (M0363)
- Protocol for Taq DNA Ligase (M0208)
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Restriction Endonucleases: Molecular Cloning and Beyond
- Molecular Cloning Technical Guide
- Synthetic Biology/DNA Assembly Selection Chart
- Troubleshooting Guide for Cloning
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
- Phelan VV, Moree WJ, Aguilar J, Cornett DS, Koumoutsi A, Noble SM, Pogliano K, Guerrero CA, Dorrestein PC (2014) Impact of a transposon insertion in phzF2 on the specialized metabolite production and interkingdom J Bacteriol; 196(9), 1683-93. PubMedID: 24532776, DOI: 10.1128/JB.01258-13
- Chinnici JL, Fu C, Caccamise LM, Arnold JW, Free SJ (2014) Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy PLoS One; 9(10), e110603. PubMedID: 25333968, DOI: 10.1371/journal.pone.0110603
- Guilinger JP, Thompson DB, Liu DR (2014) Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification Nat Biotechnol; 32(6), 577-82. PubMedID: 24770324, DOI: 10.1038/nbt.2909
- Gutjahr A, Xu SY (2014) Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA Nucleic Acids Res; 42(9), e77. PubMedID: 24609382, DOI: 10.1093/nar/gku192
- Horii T, Arai Y, Yamazaki M, Morita S, Kimura M, Itoh M, Abe Y, Hatada I (2014) Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering Sci Rep; 4, 4513. PubMedID: 24675426, DOI: 10.1038/srep04513
- Royce LA, Boggess E, Fu Y, Liu P, Shanks JV, Dickerson J, Jarboe LR (2014) Transcriptomic analysis of carboxylic acid challenge in Escherichia coli: beyond membrane damage PLoS One; 9(2), e89580. PubMedID: 24586888, DOI: 10.1371/journal.pone.0089580
- Lipscomb GL, Schut GJ, Thorgersen MP, Nixon WJ, Kelly RM, Adams MW (2014) Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane-bound complex J Biol Chem; 289(5), 2873-9. PubMedID: 24318960, DOI: 10.1074/jbc.M113.530725
- Law SH, Sargent TD (2014) The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype PLoS One; 9(6), e100268. PubMedID: 24945275, DOI: 10.1371/journal.pone.0100268
- Li Y, Thompson CM, Lipsitch M (2014) A Modified Janus Cassette (Sweet Janus) to Improve Allelic Replacement Efficiency by High-Stringency Negative Selection in Streptococcus pneumoniae PLoS One; 9(6), e100510. PubMedID: 24959661, DOI: 10.1371/journal.pone.0100510
- Gai CS, Lu J, Brigham CJ, Bernardi AC, Sinskey AJ (2014) Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16 AMB Express; 4(1), 2. PubMedID: 24410804, DOI: 10.1186/2191-0855-4-2
- Schöner TA, Fuchs SW, Reinhold-Hurek B, Bode HB (2014) Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72 PLoS One; 9(3), e90922. PubMedID: 24618669, DOI: 10.1371/journal.pone.0090922
- Vandergaast R, Hoover LI, Zheng K, Fredericksen BL (2014) Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor Viruses; 6(4), 1637-53. PubMedID: 24721788, DOI: 10.3390/v6041637
- Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA (2014) Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain PLoS Pathog; 10(2), e1003966. PubMedID: 24586168, DOI: 10.1371/journal.ppat.1003966
- Ikmi A, Gaertner B, Seidel C, Srivastava M, Zeitlinger J, Gibson MC (2014) Molecular evolution of the Yap/Yorkie proto-oncogene and elucidation of its core transcriptional program Mol Biol Evol; 31(6), 1375-90. PubMedID: 24509725, DOI: 10.1093/molbev/msu071
- Ikmi A, Gaertner B, Seidel C, Srivastava M, Zeitlinger J, Gibson MC (2014) Molecular evolution of the Yap/Yorkie proto-oncogene and elucidation of its core transcriptional program Mol Biol Evol; 31(6), 1375-90. PubMedID: 24509725, DOI: 10.1093/molbev/msu071
- DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM (2013) Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems Nucleic Acids Res; 41(7), 4336-43. PubMedID: 23460208, DOI: 10.1093/nar/gkt135
- Guye P, Li Y, Wroblewska L, Duportet X, Weiss R (2013) Rapid, modular and reliable construction of complex mammalian gene circuits Nucleic Acids Res; 41(16), e156. PubMedID: 23847100, DOI: 10.1093/nar/gkt605
- Ng S, Ivanova A, Duncan O, Law SR, Van Aken O, De Clercq I, Wang Y, Carrie C, Xu L, Kmiec B, Walker H, Van Breusegem F, Whelan J, Giraud E (2013) A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis Plant Cell; 25(9), 3450-71. PubMedID: 24045017, DOI: 10.1105/tpc.113.113985
- Singh R, Low ET, Ooi LC, Ong-Abdullah M, Ting NC, Nagappan J, Nookiah R, Amiruddin MD, Rosli R, Manaf MA, Chan KL, Halim MA, Azizi N, Lakey N, Smith SW, Budiman MA, Hogan M, Bacher B, Van Brunt A, Wang C, Ordway JM, Sambanthamurthi R, Martienssen RA (2013) The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICK Nature; 500(7462), 340-4. PubMedID: 23883930, DOI: 10.1038/nature12356.
- Ramirez-Peralta A, Gupta S, Butzin XY, Setlow B, Korza G, Leyva-Vazquez MA, Christie G, Setlow P (2013) Identification of new proteins that modulate the germination of spores of bacillus species J Bacteriol; 195(13), 3009-21. PubMedID: 23625846, DOI: 10.1128/JB.00257-13
- Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination Nucleic Acids Res; 41(20), e193. PubMedID: 24013562, DOI: 10.1093/nar/gkt805
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