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Performance of NEB glycosidases has been demonstrated in a selection of application notes, feature articles and publications from NEB scientists. Visit the publications tab at NEBGlycosidase.com to find additional citations using NEB glycosidases.

NEB application notes
featuring PNGase F

Rapid™ PNGase F

AppNote_Icon  Unbiased and fast IgG deglycosylation for accurate N-glycan analysis using Rapid PNGase F
Paula Magnelli
AppNote_Icon  Proteomics: Fast and Efficient Antibody Deglycosylation using Rapid PNGase F
Paula Magnelli and Colleen McClung

PNGase F

AppNote_Icon  Characterization of Glycans from Erbitux®, Rituxan® and Enbrel® using PNGase F (Glycerol-free), Recombinant
Beth McLeod
AppNote_Icon  Glycan Analysis of Murine IgG2a by Enzymatic Digestion with PNGase F and Trypsin, Followed by Mass Spectrometric Analysis
Alicia Bielik, Colleen McClung and Cristen Ruse

Remove-iT PNGase F

AppNote_Icon  Remove-iT PNGase F: Effective Release and Recovery of Neutral and Sialylated N-glycans
Alicia Bielik and Paula Magnelli

Select publications from NEB scientists
featuring PNGase F

NglycanComposition_thumb  N-Glycan Composition Profiling for Quality Testing of Biotherapeutics
Christopher H. Taron and Rebecca Duke
Glycomics_thumb  Glycomics: A rapidly evolving field with a sweet future
Christopher H. Taron and Pauline M. Rudd
CTaron_BehindPaper_miniThumb  Behind the paper with Chris Taron
Carbohydrates_thumb  The Structure, Function and Importance of Carbohydrates
Alicia Bielik and Paula Magnelli
Glycosidases_thumb  Using Glycosidases to Remove, Trim, or Modify Glycans on Therapeutic Proteins
Ellen P. Guthrie and Paula E. Magnelli

Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris
Genny Degani1, Alberto Barbiroli2, Paula Magnelli3, Stefania Digiovanni1, Alessandra Altomare4, Giancarlo Aldini4, Laura Popolo1

Author affiliations:

  1. Department of Biosciences (DBS) University of Milan, Milan, Italy
  2. Department of Food, Environmental and Nutritional Sciences (DeFENS) University of Milan, Milan, Italy
  3. New England Biolabs, Inc. Ipswich, MA USA
  4. Department of Pharmaceutical Sciences (DISFARM) University of Milan, Milan, Italy




One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain application.

Available PNGase F products:

NEBNext_portal_product_icon  Rapid PNGase F 
  • Rapid PNGase F (NEB #P0710) completely deglycosylates antibodies and immunoglobulin fusion proteins in 10 minutes
  • Rapid PNGase F (non-reducing format) (NEB #P0711) completely deglycosylates in 10 minutes, while preserving disulfide bonds and quaternary structure for intact antibody analysis
  • Compatible with downstream HPLC and mass spectrometry analysis
  • Recombinant enzyme, releases N- glycans rapidly and without bias


NEBNext_portal_product_icon  PNGase F, Recombinant 


NEBNext_portal_product_icon  Remove-iT™ PNGase F 
  • Remove-iT PNGase F (NEB #P0706) is CBD-tagged for easy removal of the enzyme by incubation with chitin magnetic beads
  • Identical specificity and activity as non-tagged PNGase F


NEBNext_portal_product_icon  PNGase F