Q5® Hot Start High-Fidelity 2X Master Mix
cloned at NEB recombinant No hifi hotstart pcr 3

Featured in the ARTIC SARS-CoV-2 sequencing workflow (1) and a component in the NEBNext® ARTIC SARS-CoV-2 kits.

Fidelity at its finest – for over 10 years

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Bulk format available: M0494X = 1 x 12.5 ml bottle

1. Josh Quick 2020. nCoV-2019 sequencing protocol v3 (LoCost). protocols.io https://www.protocols.io/view/ncov-2019-sequencing-protocol-v3-locost-bh42j8ye


We're sorry, this butterfly has already been found. Learn more about how NEB is celebrating our 50th anniversary.

Catalog # Concentration Size List Price Your Price Quantity
M0494S 2 X 100 reactions $245.00
M0494L 2 X 500 reactions $974.00
M0494X 2 X 500 reactions $974.00
Please enter a quantity for at least one size

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