The heterogeneity of RNA molecules in any given cell supports its ability to play a role in a number of different biological processes. Quantifying the presence of any given distinct RNA or RNA species is of obvious interest. Analysis of genome-wide differential RNA expression provides invaluable insight into biological pathways, such as regulation of gene expression. Elucidating the various roles of RNA is a crucial step in gaining a better understanding of cell biology and a number of human pathologies.
Various processing and analysis methods are needed to delineate the multifaceted expression and function of RNA. This molecular tool box provides scientists with the ability to optimize protocols for the collection of high quality data.