RNA Analysis

RNA Probe Generation

Detection of specific nucleic acid sequences can be accomplished by hybridization with a labeled RNA probe. RNA probes are sequences of a variable length that are used to detect the presence of complementary nucleotide sequences in a sample. RNA probes are first labeled with radioactive nucleotides or with modified nucleotides that can be detected by fluorescence or chemiluminescence (1). RNA probes can be used for Northern blotting, RNase protection assays, Southern blotting, downstream of polymerase chain reaction (PCR), and in situ hybridization analysis.


  1. Cooper, Geoffery M. The Cell: A Molecular Approach. 4th ed. Washington D.C.: ASM Press 2007. 131-2.
New England Biolabs supports RNA research by providing the tools necessary for synthesis, processing, transfection, detection, isolation, analysis, amplification and cloning of RNA molecules.

Protocols for RNA Probe Generation

RNA Polymerase Selection Chart

NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.