RNA Analysis

RNA Characterization & Modification

The diverse complement of cellular RNA species arises from a number of different biosynthetic pathways and can have very diverse and specialized functions. Reflecting functional and biosynthetic diversity are covalent RNA modifications that distinguish RNA classes and contribute to their functional roles.

  1. In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

Protocols for RNA Characterization & Modification

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

RNA Reagents Overview

Reagents for RNA Sample Preparation

NEB offers a wide variety of reagents to facilitate sample preparation for downstream applications including next-generation sequencing, library construction and microarrays.

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.