RNA Analysis
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  • RNA Characterization & Modification

    The diverse complement of cellular RNA species arises from a number of different biosynthetic pathways and can have very diverse and specialized functions. Reflecting functional and biosynthetic diversity are covalent RNA modifications that distinguish RNA classes and contribute to their functional roles.

    • In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

      This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

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    RNA Reagents Overview

    Reagents for RNA Sample Preparation

    NEB offers a wide variety of reagents to facilitate sample preparation for downstream applications including next-generation sequencing, library construction and microarrays.

    RNA Ligase Selection Chart

    NEB offers a variety of ligases for RNA research with unique specificities.