Many promoter systems have been developed where target gene transcription relies on the endogenous E. coli RNA polymerase. For example, IPTG-inducible promoters include the wt-lac promoter and the tac and trc promoters are lac derivatives with greater induction potential. The phage T5 promoter is also recognized by E. coli RNA polymerase: vectors employing the T5-lacO promoter include a lac operator site for expression control by the lac repressor. T5-lacO vectors typically do not encode the lacI repressor gene and therefore the expression host must supply the lac repressor. NEB Express Iq was designed specifically for IPTG-inducible vector systems where the lacI gene is not encoded by the vector.
The NEB Express strain is a multiple purpose expression strain capable of robust expression from lac, tac, trc, arabinose-inducible and rhamnose-inducible promoters. Importantly, NEB Express is recommended as the host for any pMAL vector designed for MBP-fusion protein expression.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.