Protein Expression in E. Coli
Choose Type:
- 5 Minute Transformation (C2523)
- 5 Minute Transformation Protocol (C2527)
- 5 Minute Transformation Protocol (C2528)
- 5 Minute Transformation Protocol (C2529)
- 5 Minute Transformation Protocol (C2530)
- 5 Minute Transformation Protocol (C2566)
- 5 Minute Transformation Protocol (C3010)
- 5 Minute Transformation Protocol (C3013)
- 5 Minute Transformation Protocol (C3026)
- 5 Minute Transformation Protocol (C3028)
- 5 Minute Transformation Protocol (C3029)
- 5 Minute Transformation Protocol (C3030)
- 5 Minute Transformation Protocol (C3037)
- Expression Using SHuffle (C3026)
- Expression Using SHuffle (C3028)
- Expression Using SHuffle (C3029)
- Expression Using SHuffle (C3030)
- Expression Using NEB Express (C2523)
- Protocol for Expression Using NEB Express Iq (C3037)
- Protocol for Expression Using T7 Express (C2566)
- Protocol for Expression Using T7 Express lysY (C3010)
- Protocol for Expression Using T7 Express lysY/Iq (C3013)
- Protocol for Protein Expression Using BL21 (C2530)
- Protein Expression with T7 Express strains
- Protein Expression Using Lemo21(DE3) (C2528)
- Protein Expression Using BL21(DE3) (C2527)
- Protein Expression Using NiCo21(DE3) (C2529)
-
Over 40 years in protein expression and purification – a historical perspective
This article provides an overview of the advances in protein expression and purification methodology over the past 40 years.
Feature Articles

E. coli Hosts



Disulfide bond formation in the cytoplasm of wild type E. coli is not favorable, while SHuffle is capable of correctly folding proteins with multiple disulfide bonds in the cytoplasm.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.