Protein Expression Products

New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress® products, resulting from our 40+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.

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Protein Expression
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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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E. coli Ribosome

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Factor Xa Protease

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IMPACT™ Kit

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K. lactis GG799 Competent Cells

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K. lactis Protein Expression Kit

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Lemo21(DE3) Competent E. coli

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NEBExpress® Cell-free E. coli Protein Synthesis System

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® GamS Nuclease Inhibitor

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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NiCo21(DE3) Competent E. coli

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pMAL-c6T Vector

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pTXB1 Vector

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pTYB21 Vector

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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RNase Inhibitor, Murine

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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TEV Protease

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Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

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E. coli Ribosome

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NEBExpress® Cell-free E. coli Protein Synthesis System

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NEBExpress® GamS Nuclease Inhibitor

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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RNase Inhibitor, Murine

Protein Expression in Yeast

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K. lactis GG799 Competent Cells

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K. lactis Protein Expression Kit

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Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

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Factor Xa Protease

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Lemo21(DE3) Competent E. coli

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NEBExpress® MBP Fusion and Purification System

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pMAL-c6T Vector

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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TEV Protease

Protein Expression in E. Coli

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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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IMPACT™ Kit

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Lemo21(DE3) Competent E. coli

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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NiCo21(DE3) Competent E. coli

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pMAL-c6T Vector

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pTXB1 Vector

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pTYB21 Vector

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Cell-Free Protein Expression
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E. coli Ribosome

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NEBExpress® Cell-free E. coli Protein Synthesis System

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NEBExpress® GamS Nuclease Inhibitor

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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RNase Inhibitor, Murine

NEBExpress® Cell-free E. coli Protein Synthesis System

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NEBExpress® Cell-free E. coli Protein Synthesis System

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NEBExpress® GamS Nuclease Inhibitor

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PURExpress® Disulfide Bond Enhancer

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RNase Inhibitor, Murine

PURExpress

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E. coli Ribosome

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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RNase Inhibitor, Murine

Protein Expression in Yeast
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K. lactis GG799 Competent Cells

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K. lactis Protein Expression Kit

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Yeast Carbon Base Medium Powder

Expression of Difficult Proteins
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Lemo21(DE3) Competent E. coli

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NEBExpress® MBP Fusion and Purification System

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pMAL-c6T Vector

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Disulfide-bonded Protein Expression

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PURExpress® Disulfide Bond Enhancer

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PURExpress® In Vitro Protein Synthesis Kit

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

Membrane Protein Expression

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Lemo21(DE3) Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Toxic Protein Expression

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Lemo21(DE3) Competent E. coli

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PURExpress® In Vitro Protein Synthesis Kit

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Target Protein Insolubility

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Factor Xa Protease

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NEBExpress® MBP Fusion and Purification System

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pMAL-c6T Vector

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TEV Protease

Protein Expression in E. Coli
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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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IMPACT™ Kit

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Lemo21(DE3) Competent E. coli

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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NiCo21(DE3) Competent E. coli

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pMAL-c6T Vector

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pTXB1 Vector

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pTYB21 Vector

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

T7 Expression

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BL21(DE3) Competent E. coli

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Lemo21(DE3) Competent E. coli

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NiCo21(DE3) Competent E. coli

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Non-T7 Expression

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BL21 Competent E. coli

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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pMAL-c6T Vector