Target Protein Insolubility
Choose Type:
- NEBExpress MBP Fusion and Purification System Quick Start Protocol (NEB #E8201)
- Cloning a PCR Fragment Into a pMAL Expression Vector (NEB #E8201)
- NEBuilder Assembly of a PCR Fragment
- Transformation Protocol (NEB #E8201)
- Small Scale Affinity Chromatography (NEB #E8201)
- Large Scale Affinity Chromatography (NEB #E8201)
- Cleavage of the Fusion Protein
- Separating the Protein of Interest from MBP after TEV Protease Cleavage (NEB #E8201)
- E. coli Lemo21(DE3)
- Protein Expression with T7 Express Strains
- Transformation of SHuffle® Competent Cell Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- DNA Sequences and Maps Tool
- Competent Cell Product Comparison
- Protein Expression and Purification Selection Chart
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For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
