Expression of Difficult Proteins
Choose Type:
- What are the strain properties of Lemo21(DE3) competent E. coli?
- Where can I find more detailed FAQs for the K. lactis Protein Expression Kit?
- What systems does NEB offer for protein expression and purification?
- Which SHuffle® strain should I use?
- How do SHuffle® strains aid in cytoplasmic disulfide bond formation?
- What is LysY?
- What applications are SHuffle® strains useful for?
- High Efficiency Transformation Protocol
- Protein Expression with T7 Express strains
- Expression Using SHuffle (C3030)
- 5 Minute Transformation Protocol (C3026)
- High Efficiency Transformation Protocol
- 5 Minute Transformation Protocol (C3030)
- 5 Minute Transformation Protocol (C3029)
- Expression Using SHuffle (C3026)
- Expression Using SHuffle (C3029)
- E. coli Lemo21(DE3)
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Protein Expression with T7 Express Strains
- Transformation of SHuffle® Competent Cell Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
-
Over 40 years in protein expression and purification – a historical perspective
This article provides an overview of the advances in protein expression and purification methodology over the past 40 years.
-
The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
-
Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Competent Cell Product Comparison
- Protein Expression and Purification Selection Chart
- Convenient Formats of Competent Cells
Feature Articles
Brochures
Selection Tools
Usage Guidelines

B) Soluble fractions of B. malayi protein expressed at 30°C in Lemo21 (DE3).


This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.