There are many choices when selecting a method for expressing a recombinant protein. In general, common expression systems used in life sciences research are microbial (bacteria or yeast), higher eukaryotic (insect or mammalian cells) or cell-free (in vitro protein expression). Because of their speed, low cost, and ability to be easily genetically modified, microbes are often a first choice for in vivo expression of a target protein. For expression in bacteria, E. coli has been the most widely utilized used host for almost 25 years. Similarly, expression in various yeasts has been common since the mid-1980’s. Finally, production of proteins in vitro in cell-free systems is often used in situations where a target protein is toxic to a host or introduction of non-native amino acids into the protein’s structure is desired.
Protein Expression Approaches includes these areas of focus:
FAQs for Protein Expression Approaches
Protocols for Protein Expression Approaches
- E. coli Lemo21(DE3)
- Protein Expression with T7 Express Strains
- Transformation of SHuffle® Competent Cell Strains
Competent Cells Brochure
The Competent Cells brochure provides information on the different competent cell strains for cloning and protein expression available from NEB.
Protein Expression & Purification Brochure
The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.
Bypassing Common Obstacles in Protein Expression
The Next Generation of Reagents for Sample Preparation
Why Choose the K. lactis Protein Expression Kit?
- DNA Sequences and Maps Tool
- Competent Cell Product Comparison
- IMPACT™ Vectors and Applications
- Protein Expression and Purification Selection Chart
- Convenient Formats of Competent Cells
Other Tools & Resources
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.