There are many choices when selecting a method for expressing a recombinant protein. In general, common expression systems used in life sciences research are microbial (bacteria or yeast), higher eukaryotic (insect or mammalian cells) or cell-free (in vitro protein expression). Because of their speed, low cost, and ability to be easily genetically modified, microbes are often a first choice for in vivo expression of a target protein. For expression in bacteria, E. coli has been the most widely utilized used host for almost 25 years. Similarly, expression in various yeasts has been common since the mid-1980’s. Finally, production of proteins in vitro in cell-free systems is often used in situations where a target protein is toxic to a host or introduction of non-native amino acids into the protein’s structure is desired.
Bypassing Common Obstacles in Protein Expression
The Next Generation of Reagents for Sample Preparation
Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
Competent Cells Brochure
The Competent Cells brochure provides information on the different competent cell strains for cloning and protein expression available from NEB.
Protein Expression & Purification Brochure
The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.
- DNA Sequences and Maps Tool
- Competent Cell Product Comparison
- IMPACT™ Vectors and Applications
- Protein Expression and Purification Selection Chart
- Convenient Formats of Competent Cells
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.